Gene targeting strategy for B-hICAM1 mice. The exons 2-7 of mouse Icam1 that encode the full-length protein, not including signal peptides were replaced by the exons 2-7 of human ICAM1 gene in B-hICAM1 mice.

Strain specific ICAM1 expression analysis in homozygous B-hICAM1 mice by flow cytometry. T cells and B cells from the spleen were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hICAM1 mice (H/H), and analyzed by flow cytometry with species-specific anti-ICAM1 antibodies. Mouse ICAM1 was detectable in wild-type mice. Human ICAM1 was exclusively detectable in homozygous B-hICAM1 mice but not in wild-type mice.

Strain specific ICAM1 expression analysis in homozygous B-hICAM1 mice by flow cytometry. NK cells, dendritic cells from the spleen were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hICAM1 mice (H/H), and analyzed by flow cytometry with species-specific anti-ICAM1 antibodies. Mouse ICAM1 was detectable in wild-type mice. Human ICAM1 was exclusively detectable in homozygous B-hICAM1 mice but not in wild-type mice.

Strain specific ICAM1 expression analysis in homozygous B-hICAM1 mice by flow cytometry. Granulocytes, monocytes, and macrophages from the spleen were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hICAM1 mice (H/H), and analyzed by flow cytometry with species-specific anti-ICAM1 antibodies. Mouse ICAM1 was detectable in wild-type mice. Human ICAM1 was exclusively detectable in homozygous B-hICAM1 mice but not in wild-type mice.